Screening of endophytic fungi as potential antagonistic agents of Pyricularia oryzae and evaluation of their ability in producing hydrolytic enzymes

Abstract. Putri ND, Sulistyowati L, Aini LQ, Muhibuddin A, Trianti I. 2022. Screening of endophytic fungi as potential antagonistic agents Pyricularia oryzae and evaluation their ability in producing hydrolytic enzymes. Biodiversitas 23: 1048-1057. One the most important phytopathogenic is oryzae, it causative agent rice blast diseases, which destructive detrimental disease rice. Biological control using can be an alternative to diseases. Endophytic are that colonize internal plant tissues without apparently damaging host. The aim this study was isolate produce enzymes investigate suppress disease. In study, fifty strains were successfully obtained from leaves. Three with highest inhibition against P. identified Trichoderma asperellum, Curvularia chiangmaiensis, Fusarium solani by analyzing fungal ITS sequence. They chitinase ranging 1.43 151 ?g/mL cellulase 1.83 4.09 ?g/mL, account for lysis phytopathogens. This enzymatic activity cause damage degradation cell walls. SEM results revealed these probably excreted wall lytic or antifungal substances, inflicting wrinkles disintegrating mycelium.